Identification of Escherichia coli by analysis of 16S rRNA and study some Virulence genes isolated from female genital tract Infections

  • Rahman Laibi Chelab Department of Biology, College of Education for Pure Sciences, Thi-Qar University
  • Enas Razaq ALwaeli Department of Anesthesia Techniques, College of Medical and Health Technology, AL-Ayen University.
  • Enaas Saleh Jawad Department of Gynecology and obstetrics. Medical college. Thi-Qar University
Keywords: vaginitis, Escherichia coli, antimicrobial susceptibility, virulence genes

Abstract

        Vaginal diseases such as trichomoniasis, vulvovaginal candidiasis (VVC) and bacterial vaginosis (BV) are widespread worldwide. Accurate detection and administration of effective medications are essential, since such infections are related to negative effects for women during pregnancy and for newborns However, the presence of Escherichia coli in such infections is controversial, and hence it is important to identify these genetic and typical characteristics of E. coli strains isolated from genital infection, this review will focus on the diagnosis of E. coli isolates by 16SrRNA, discovering the effectiveness of some antimicrobials and studying some genes of virulence factors that affect the female reproductive system, In addition to its effect on fetuses in pregnant women. include with its impression share (fimH), type 1 fimbriae (%76.36), (cnf1), cytotoxic ,necrotizing factor (36.36%), (usp) uropathogen specific protein (58.18%) and (ibeA), invation brain endothelium (32.72%), from (300) female genital tract secretions specimens isolate ,The number of bacterial isolates was 165 (55%) and the number of yeast isolates was 110 (36,6%) no growth 25(8,33%) and number of E.coil isolates(55) ,The results also showed that the resistance and sensitivity of the different types of antimicrobial such as ,amoxicillin-clavulanic acid(AMC) (54.54%) doxycycilin (DO) (32.72%) ,cefixime (CFM)(54.54%) ceftriaxone(CRO)(50.90%), cephalothin (KF) (14.54%) ,Cefotaxime (CTX) (54.54%) ,Nitrofurantoin (NI) (9.09%) ,ciprofloxacin (CIP) (78.18%) , gentamicin (CN) (41.81%) trimethoprim /sulfamethoxazole (TS) (27.27%),imipenem(IPM) (89.09%) meropenem,(MEM) (90.90%), Amikacin (AK) (58.18%) ,Netilmicin (NET) (32.72%) ,lincomycin (L) ,(14.54%) Tobramycin (TN) (20%).         

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Published
2021-02-18
Section
Articles