Detecting of Chemical compounds of aqueous and alcoholic extracts of damasConocarpus lancifolus Engl.leaves using GC-MS technique

Authors

  • college of Science University of Basrah
  • College of Science University of Basrah

DOI:

https://doi.org/10.32792/jeps.v12i1.143

Keywords:

Chemical compounds, damas leave, GC-MS

Abstract

This stady was conducted to identify the chemical compounds in the aqueous and alcoholic extract of
damas leaves Conocarpus lancifolus Engl. using the GC-MS equipment on instrument . The results of
this study showed the aqueous extract contained 23 chemical compounds and the alcoholic extract
contained 22 chemical compounds. The compounds varied many alkaloids such us Dicyclopentadiene
diepoxide, glycosides such us Lactos , terpenes such us alpha-Amyrin, fatty acids such us Oleioc acid,
esters such us Hexadecanoic acid, ethyl ester and phenolic such us Pluchidiol compounds. The compound
(.alpha.,.beta.-Gluco-octonic acid lactone) recorded the highest percentage, while the compound
(Dicyclopentadiene diepoxide) recorded the lowest percentage in the aqueous extract, while in the
alcoholic extract (gamma.-Sitosterol) that the actieved the highest percentage, and the compound
(Bicyclo] 2.1.1]hexan-2-ol,2-ethenyl) is the lowest.The similarity between the aqueous and alcoholic
extracts in 5 compounds (Thiosulfuric acid (H2S2O3), S-(2-aminoethyl) ester, L-Serine O-
(phenylmethyl),Malonic acid hept -6- ynyl , 1-Heptatatriacotanol, 2,7-Diphenyl- 1,6-
dioxopyridazino[4,5:2,3]pyrrolo[4,5-d]pyridazine). Most of the existing compounds had a known medical
importance that varied between anti-cancer such as (alpha.-Amyrin) and antibiotics such as (gamma.-
Sitosterol) and some of them used in treatment in cardiac systems such as (Procainamide

References

Abd Ali B. A. (2013). An environmental study on the conocarpus tree Conocarpus lancifolius Engl.

Newly entered to Iraq. Research entrance and museum of natural history – Baghdad University. 2(207).(

in arabic)

Akpuaka, A.; Ekwenchi, MM. and Dashak, D. A. (2013). Biological Activities of Characterized Isolates

of n-Hexane Extract of Azadirachta Indica A. Juss (Neem) Leaves. Nature and Science, 11(5):141-147

AL- Fakiki, D. F. A. 2013. Determination of the fatty acids of the oil from the kernels of four date palm

cultivars (Phoenix dactylifera L.) using gas chromatography-connected mass spectrometry (GC-MS).

Basra Journal of Date Palm Research, Vol. 12, p. 1-2, p. 16-30.https://search.emarefa.net/detail/BIM-

( in arabic)

AL-Dabbagh ,R. A. (2013). The damas tree whats for it and whats on it .Articale in ALKhaleej Emirates

Newspaper( in Arabic)

AL-Fakiki D. F. A. (2013). Extraction, purification and characterization of the alpha-amylase enzyme

extracted from germinated sorghum kernels. Basra Research Journal: Operations, Vol. 39, p. 1b, pp. p.

-26. ( in arabic)

Al-Kinani A. H. A. (2020). Molecular and chemical study of some types of medicinal plants of the

labiatae family with an explanation of their role as antioxidants. Master's thesis. University of Basra.

College of Science. biology Sciences.

Al-Maliki H. H. N. (2012) The use of the Conocarpus lancifolius Engl. As a bioaccumulator of some

heavy metals in Basra Governorate. Higher diploma thesis. Albasrah university . Faculty of Science

Department of Environment( in arabic)

Al-Saad O. A. L. (2020) A chemical and molecular study of some species of the brassicaceae family and

Al-Saad O. A. L. (2020) A chemical and molecular study of some species of the brassicaceae family and

the effect of glycoside extracts and antioxidants. Master Thesis . College of Science . Department of

biology. ( in arabic)

AL-Satti ,H.A.;Amina, R. ;Patrice, S. and Redh, A. (2014) .The Allelopathic Potentiol of Conocarpus

lancifolius (Engl.) Leaves on dicot (Vigna sinensis L.),Monocot (Zea mays L.)and soil –Borne Pathogenic

Fungi. American J.of plant science.2889-2903.

Aneesh S.(2013). Asian J Pharm Clin Res,; 6(3):68-74.

Arora K. (2017). Screening and evaluation of bioactivecomponents of Cenchrus ciliaris L. by GC-MS

analysis.International Research Journal of Pharmacy 8 (6): 69-76.

Butler L. I.; McDonough L. M. (1981) Insect sex pheromones: Evaporation rates of alcohols and acetates

from natural rubber septa. Journal of Chemical Ecology. 7(3):27–633.

Chandler, S. E.(1982) . Letter ex Imperial Institute, London accompainying Kew Herbarium specimen .

Quot. From Booth and Wickens,1988.

Chandrasekaran, M.; Senthilkumar, A. and Venkatesalu, V.(2011). Antibacterial and antifungal efficacy

of fatty acid methyl esters from leaves of Sesuvium portulacastrum L. Eur. Rev. Med. Pharmcol. Sci., v.

, no. 7, p. 775-780.

Chitra S. and Karthikeyan J.( 2018). Phytochemical profiling of act whiskers ( orthosiphon stamineus )

tea leaves extract . Jorunal of pharmacognous and phytochemistry ; 7(6):1396-1402 .

Dilika, F.; Bremner, PD. and Meyer JJM. (2000). Antibacterial activity of linoleic and oleic acids isolated

from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia, 71(4):450-452.

Dinesh kumar, G. and Rajakumar, R.(2016). Gas Chromatography-Mass Spectrometry Analysis of

Bioactive Components from the Ethanol Extract of Avicennia marina Leaves. Innovare Journal of

Science; 4(4):9-12.

Harborne , J.B. ( 1984 ) . Phytochemical methods : Aguide to modern techniques of plant analysis.

Chapman and Hall . London pp 278 .

Hema, R.; Kumaravel, S.; Alagusundaram, K.(2011). GC/MS determination of bioactive components of

Murraya koenigii. Journal of American Science, v. 7, no. 1, p. 80-83

International Plant Names Index (IPNI) Copyright (2005 ).

IUCN, (2012 ). IUCN Red List of Threatened Species. Version 2012.2. .

Katab Zyada .2020 . what do you Knowabout his nutritional value .

Kavitha R. (2021). Phytochemical screening and GC-MS analysis of bioactive compounds present in

ethanolic extracts of leaf and fruit of trichosanthesis dioica roxb. International Journal of Pharmaceutical

Sciences and Research.DOI: 10.13040/IJPSR.0975-8232.12(5).2755-64.

Krishnamoorthy, K.; Subramaniam, P.(2014) Phytochemical profiling of leaf, stem, and tuber parts of

Solena amplexicaulis (Lam.) Gandhi using GC-MS. InternationalScholarly Research Notices, v, Article

Kubo, I.; Muroi, H. and Kubo A. (1994). Naturally Occurring Antiacne Agents. Journal of Natural

Products, 57(1):9-17.

Mahmood I. Sh., (1993) ,Trees of Pakistan . 142 pp pdf.usaid.gov/pdf_docs/pnabw250.pdf

Malik, S. ;Muhammad, A. ;Bashir, A. Ch.; Hafiza, S. Y. ;Muhammed, U. and Khurram, A.

(2020).Isolation characterizathion and preliminary cytotoxic and antifungal evaluations of novel

Lancifoliate isolated form methanol extract of Conocarpus lancifolius. Anti- Cancer Agents in Medicianal

Chemistry . . 20-14. DIO : 10.2174/1871520620620666200424110923

Malik, S. ;Muhammed, A. ;Shabana, B. ;Muhammed, A. S. ;Tahir, A.C.; Azhar, R.; Muhammad, U

;Mohammed, A.; Sara, T. E. and Gabar E. (2021) .Phytochemical and Molecular Dynamic Analysis

ofNovel Biomolecule Lancifotarene Extracted From Conocarpus Lancifolius as Cytotoxic, Antiureaseand

Antidiabetic Agent . DOI:

Mangunwidjaja, D. S.; Kardono, S. R. and Iswantini, L. B. S. D.(2006) Gas chromatography and gas

chromatography-mass spectrometry analysis of Indonesian Croton tiglium seeds. J.AppliedSci., v. 6, no.

, p. 1576-1580.

Medlool Narges Kazem (2021). Histological and physiological study of the effect of the good plants

cynomoruum co cinema and lichen (distance tubulosa) on testes of laboratory mice. Master's thesis,

College of Science. Department of biology( in arabic)

Mellon, F.(2000) Mass Spectrometry of Natural Substances in Foods. RSC Bood Analysis Monograps,

Ed. Belton. The Royal Society Chemistry Cambridge, U.K.

Mirbahar, M. B.and Yaseen ,S. M.,(1996). Disposal of saline drainage water in agro-forestry systems.

Proceedings of th drainage workshop on drainage and the environment, Ljubljana, Slovenia, April21

,1996 . pp.497 -504.

Mohammed, Y. H.;Ghaidaa, J. M. and Iman, H. H. 2016.Analysis of bioactive chemical compounds of

Nigella sativa using gas chromatography-mass spectrometry . Journal of Pharmacognosy and

Phytotherapy.2141-2502 .DOI: 10.5897/JPP2015.0364 .

Olaleye, OO.; Kukwa, RE.; Eke, MO. and Aondo ,TO. (2018).Extraction, Physicochemical and

PhytochemicalCharacterization of Oil from Sesame Seed. Asian FoodScience Journal 1 (4): 1-12.

Rajeswari, B. and Srinivasan, M.(2015).GC-MS analysis of bioactive components from the ethanolic leaf

extract of Flueggea leucopyrus Wild. International Journal of Pharmaceutical Sciences Review and

Research, v. 33, no. 1, p. 270-273.

Rajeswari, G.; Murugan, M. and Mohan, V. R.(2012) GC-MS analysis of bioactive components of

Hugonia mystax L. (Linaceae). Research Journal of Pharmaceutical, Biological and Chemical Sciences,

v. 3, no. 4, p. 301-308.

Ruba otoom .(2021). Procainamide mediciane .

Selvan, P. S. and Velavan, S.(2015) Analysis of bioactive compounds in methanol extract of Cissus

vitiginea leaf using GC-MS technique. Rasayan Journal of Chemistry, v. 8, no. 4, p. 443-447.

Suleman, P., Redha, A., Afzal, M. and Al-Hasan, R. (2013) Temperature-Induced Changes of

Malondialdehye, Heat-Shock Proteins in Relation to Chlorophyll Fluorescence and Photosynthesis in

Conocarpus lancifolius (Engl.). Acta Physiologiae Plantarum, 35, 1223-1231.

Venkata, R.; Samuel, L.; Pardha ,SM.; Narasimhan, R.; Naga ,VKA.; Sudhakar, M. and Radhakrishnan

TM. (2012). Antibacterial, antioxidant activity and GC-MS Analysis of Eupatorium odoratum. Asian

Journal of Pharmaceutical and Clinical Research, 5(2):0974-2441

Downloads

Published

2023-01-16