Association Of Tumor Necrosis Factor- Alpha (TNF-α) Polymorphism in Renal Failure Patients


  • Department of Biology, College Education for Pure Sciences, University of Thi-Qar
  • 1Department of Biology, College Education for Pure Sciences, University of Thi-Qar
  • 1Department of Biology, College Education for Pure Sciences, University of Thi-Qar


Renal failure is a disorder in which the kidneys are unable to maintain the fluid, electrolyte, and pH
balance of the extracellular fluids as well as remove metabolic waste products from the blood. The current
study's objective was to investigate the relationship between the TNF-α promoter -264 C/T single nucleotide
polymorphism (SNP) ,and glomerular filtration rate (GFR) in renal failure with and without diabetic mellitus
patients compared to control. This study was conducted in the laboratories of Thi-Qar University's College
of Education for Pure Sciences in collaboration with Al-Hussein Teaching Hospital, Nasiriyah General
Hospital, and Thi-Qar Center for Nephrology and Dialysis in Thi-Qar Province from February 2022 to
March 2023. The study included 40 renal failure with D.M patients, 40 patients without DM and 40 control.
By using the PCR technique, the TNF-promoter gene polymorphism -264 C/T was identified, and
genotyping was verified by direct sequencing. The current study found that there is a significant effect of
TNF-α gene in renal failure patients with and without D.M compared to control. In addition, this study
demonstrated an inverse correlation between effect of TNF-alpha gene on end-stage renal disease and
decreased eGFR. The study concluded that there was a significant effect of TNF- α gene polymorphism is
associated with markers of kidney disease severity and distant organ dysfunction among patients with RF. In
addition, this study found correlation between effect of TNF-alpha gene on end-stage renal disease and
decreased eGFR.


[ 1] Obesity, Weight Loss, and Number Sex Age Range. "Clinical Research Protocol National Institute of

Diabetes and Digestive and Kidney Diseases." (2017).

[ 2] Corsello, Andrea, et al. "Walter E. Dandy: his contributions to pituitary surgery in the context of the

overall Johns Hopkins Hospital experience." Pituitary 20 (2017): 683-691.

[ 3] Sameen, Faisal Younus, et al. "Assessment Knowledge of the patients’ with acute renal failure

concerning dietary pattern in dialysis unite of AL-Zahraa Teaching Hospital at AL-Kut city." Prof.(Dr)

RK Sharma 21.1 (2021): 249.

[ 4] Liao, Min-Tser, et al. "Insulin resistance in patients with chronic kidney disease." Journal of

Biomedicine and Biotechnology 2012 (2012).

[ 5] Bonventre, Joseph V.; WEINBERG, Joel M. Recent advances in the pathophysiology of ischemic acute

renal failure. Journal of the American Society of Nephrology, 2003, 14.8: 2199-2210.

[ 6] Akcay, Ali; Nguyen, Quocan; Edelstein, Charles L. Mediators of inflammation in acute kidney

injury. Mediators of inflammation, 2009, 2009.

[ 7] Lee, D. W.; Faubel, S.; Edelstein, CL21888852. Cytokines in acute kidney injury (AKI). Clinical

nephrology, 2011, 76.3: 165-173.

[ 8] White, Laura E.; Hassoun, Heitham T. Inflammatory mechanisms of organ crosstalk during ischemic

acute kidney injury. International journal of nephrology, 2012, 2012. .

[ 9] O'malley, W. Edward; Achinstein, Betty; Shear, Murray J. Action of bacterial polysaccharide on tumors.

II. Damage of sarcoma 37 by serum of mice treated with Serratia marcescens polysaccharide, and

induced tolerance. Journal of the National Cancer Institute, 1962, 29.6: 1169-1175.

[ 10] Hayashi, Kentaro, et al. A systems biology approach to suppress TNF-induced proinflammatory gene

expressions. Cell Communication and Signaling, 2013, 11: 1-15.

[ 11] Zhang, Bei-Bei, et al. Association between TNF α gene polymorphisms and the risk of duodenal

ulcer: a meta-analysis. Plos one, 2013, 8.2: e57167.

[ 12] Locksley, Richard M.; Killeen, Nigel; Lenardo, Michael J. The TNF and TNF receptorsuperfamilies: integrating mammalian biology. Cell, 2001, 104.4: 487-501.

[ 13] Elahi, Maqsood M., et al. Tumor necrosis factor alpha− 308 gene locus promoter polymorphism: an

analysis of association with health and disease. Biochimica et Biophysica Acta (BBA)-Molecular Basis of

Disease, 2009, 1792.3: 163-172.

[ 14] Barbara, Jeffrey AJ; Van ostade, Xaveer; LOPEZ, Angel F. Tumour necrosis factor