Effect of Cinnamomum zeylanicum Bark Extracts on Clinically Important Drug Resistant Pathogenic Bacteria


  • General Directorate of Education in Thi-Qar, Ministry of Education, Iraq Corresponding
  • General Directorate of Education in Thi-Qar, Ministry of Education, Al-Nasiriya City, Iraq
  • General Directorate of Education in salahuddin / Tikrit
  • General Directorate of Education in Thi-Qar, Ministry of Education, Al-Nasiriya City, Iraq


Background: Cinnamomum Zeylanicum, is one of the oldest herbal medicines and a popular spice crop used in Asian countries. It has been valued for centuries for its distinct flavor and aroma, and it also possesses various medicinal properties. In Cinnamomum Zeylanicum, there are many constituents such as cimmaldehyde, eugenol, linalool and cinnamic acid. Therapeutic compounds found in plants are gaining attention as a potential source for new drug development. Objectives : The study aims to identify potential novel treatments for bacterial infections by evaluating commercially available ethanolic extracts' in vitro antimicrobial activity and observing their effects on bacterial biological activity inhibition. Material and methods: The well diffusion method was used to examine the in vitro antimicrobial activity of an ethanol extract of Cinnamomum zeylanicum bark against both gram-positive (S. aureus and S.epidermides) and gram-negative (E. coli and P. aeruginosa) standard microbes. In this study, all pathogenic bacteria that affect humans tested were inhibited to varying degrees by the concentrations investigated. Results:The results indicate that the inhibition zone for gram positive bacteria is greater than that of gram negative bacteria at all concentrations, ranging from 19.22 (29%)mm to 18.44 (28%)mm against S. aureus and S. epidermids. The inhibition zones for E. coli and P. aeruginosa are 14.67 (22%) and 14.11 (21%) respectively. Conclusion: In this study, all pathogenic bacteria that affect humans were inhibited to varying degrees by the concentrations investigated.


Cao, H., Sethumadhavan, K., Li, K., Boue, S. M., & Anderson, R. A. (2019). Cinnamon polyphenol extract and insulin regulate diacylglycerol acyltransferase gene expression in mouse adipocytes and macrophages. Plant Foods for Human Nutrition, 74, 115-121.

Vijayakumar, K., Rengarajan, R. L., Suganthi, N., Prasanna, B., Velayuthaprabhu, S., Shenbagam, M., & Vijaya Anand, A. (2022). Acute toxicity studies and protective effects of Cinnamon cassia bark extract in streptozotocin-induced diabetic rats. Drug and Chemical Toxicology, 45(5), 2086- 2096.

B. Varalakshmi, A. Vijaya, K. Vijayakumar, and R. Prasanna, "In vitro antioxidant activity of Cinnamomum zeylanicum linn bark," Int J Ins Pharm Life Sci, vol. 2, no. 3, 2012.

Vasconcelos, N. G., Croda, J., & Simionatto, S. (2018). Antibacterial mechanisms of cinnamon and its constituents: A review. Microbial pathogenesis, 120, 198-203.

Albano, M., Crulhas, B. P., Alves, F. C. B., Pereira, A. F. M., Andrade, B. F. M. T., Barbosa, L. N., ... & Júnior, A. F. (2019). Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis. Microbial pathogenesis, 126, 231-238

Cheung, G. Y., Bae, J. S., & Otto, M. (2021). Pathogenicity and virulence of Staphylococcus aureus. Virulence, 12(1), 547-569.

Piri-Gharaghie, T., Jegargoshe-Shirin, N., Saremi-Nouri, S., Khademhosseini, S. H., Hoseinnezhad-Lazarjani, E., Mousavi, A., ... & Fatehi-Ghahfarokhi, S. (2022). Effects of Imipenem-containing Niosome nanoparticles against high prevalence methicillin-resistant Staphylococcus Epidermidis biofilm formed. Scientific reports, 12(1), 5140.

Chabi, R., & Momtaz, H. (2019). Virulence factors and antibiotic resistance properties of the Staphylococcus epidermidis strains isolated from hospital infections in Ahvaz, Iran. Tropical medicine and health, 47(1), 1-9.

Adamus-Bialek, W., Zajac, E., Parniewski, P., & Kaca, W. (2013). Comparison of antibiotic resistance patterns in collections of Escherichia coli and Proteus mirabilis uropathogenic strains. Molecular biology reports, 40, 3429-3435.

Karimi, S., Lotfipour, F., Asnaashari, S., Asgharian, P., Sarvari, Y., & Hazrati, S. (2019). Phytochemical analysis and anti-microbial activity of some Important medicinal plants from north-west of Iran. Iranian Journal of Pharmaceutical Research: IJPR, 18(4), 1871.

Akujobi, C., Anyanwu, B. N., Onyeze, C., & Ibekwe, V. I. (2004). Antibacterial activities and preliminary phytochemical screening of four medicinal plants. J. Appl. Sci, 7(3), 4328-4338.

Gupta, E., Siddque, S., & Khare, V. (2023). International Journal of Medical and Allied Health Sciences. Int. J. Med. & Allied Health Sci. Vol, 3(01).

Zhu, Y., Huang, W. E., & Yang, Q. (2022). Clinical perspective of antimicrobial resistance in bacteria. Infection and drug resistance, 735-746.

Ryan, E. T., Hill, D. R., Solomon, T., Aronson, N., & Endy, T. P. (2019). Hunter's tropical medicine and emerging infectious diseases e-book. Elsevier Health Sciences.

Box GE, Hunter WH, Hunter S. Statistics for experimenters: John Wiley and sons New York; 1978.

HUSSEIN, U. A. R., SALIH, H. A., ABBAS, A. T., & MTUASHER, S. M. (2020). In vitro antibacterial activity of some spice extracts against methicillin-resistant Staphylococcus aureus isolated from nose of food handlers. International Journal of Pharmaceutical Research (09752366), 12(3).

Yardimci, B. K., Sahin, S. C., Sever, N. I., & Ozek, N. S. (2022). Biochemical effects of sodium benzoate, potassium sorbate and sodium nitrite on food spoilage yeast Saccharomyces cerevisiae. Biologia, 77(2), 547-557.

Tavares, T. D., Antunes, J. C., Padrão, J., Ribeiro, A. I., Zille, A., Amorim, M. T. P., ... & Felgueiras, H. P. (2020). Activity of specialized biomolecules against gram-positive and gram- negative bacteria. Antibiotics, 9(6), 314.

Gishen, N. Z., Taddese, S., Zenebe, T., Dires, K., Tedla, A., Mengiste, B., ... & Lulekal, E. (2020). In vitro antimicrobial activity of six Ethiopian medicinal plants against Staphylococcus aureus, Escherichia coli and Candida albicans. European Journal of Integrative Medicine, 36, 101121.

Ambrosio, C. M. S., Contreras‐Castillo, C. J., & Da Gloria, E. M. (2020). In vitro mechanism of antibacterial action of a citrus essential oil on an enterotoxigenic Escherichia coli and Lactobacillus rhamnosus. Journal of Applied Microbiology, 129(3), 541-553.

Kong, A. S. Y., Maran, S., Yap, P. S. X., Lim, S. H. E., Yang, S. K., Cheng, W. H., ... & Lai, K. S. (2022). Anti-and Pro-oxidant properties of essential oils against antimicrobial resistance. Antioxidants, 11(9), 1819.

Cock, I. E., Ndlovu, N. A., & Van Vuuren, S. F. (2023). The traditional use of southern African medicinal plants to treat oral pathogens, and studies into their relevant antimicrobial properties. South African Journal of Botany, 153, 258-279.

P. Masika and A. Afolayan, "Antimicrobial activity of some plants used for the treatment of livestock disease in the Eastern Cape, South Africa," Journal of Ethnopharmacology, vol. 83, no. 1-2, pp. 129-134, 2002.