Effect of Haptoglobin Genotype on Essential Mineral Levels in Patients With Sickle Cell Anemia, Hepatitis C: A comparative Analysis

Authors

  • University of Thi-Qar/ Collage of Vet-Medicine.
  • University of Thi-Qar/ Collage of Science.
  • University of Thi-Qar/ Collage of Science.

DOI:

https://doi.org/10.32792/jeps.v13i4.378

Abstract

Abstracte
The current study was conducted in the laboratories of the Marsh Research Center, the Center for Genetic Diseases, and Al-Hussein Teaching Hospital during the period from June 2022 to January 2023, with the aim of studying the genetic polymorphism of the haptoglobin (Hp) gene and its relationship to levels of essential minerals in patients with sickle cell anemia. Hepatitis C, and sickle cell anemia with hepatitis C. a total of 130 participants were classified, (40) control groups, (40) patients with sickle cell anemia, (40) patients with hepatitis C, and (10) sickle cell patients with hepatitis C .
DNA was isolated and polymerase chain reaction (PCR) performed using genotype-specific primers for the three regions of the haptoglobin gene, and genotypes were determined after electrophoresis on agarose gels and determining the amplified fraction of each allele.Iron, copper, zinc, and chromium in blood serum were determined by flame atomic absorption spectrometry according to the manufacturer's procedures.
The study found that the Hp2-2 genotype was more common in patients with sickle cell anemia, hepatitis, and both sickle cell anemia and hepatitis compared to the healthy control group.However, there were no significant differences in this genotype between the three diseases. In all patients, the level of iron and copper in the serum was significantly higher compared to the control group.and In all patients, the study showed a significant depletion of serum zinc compared to the control group,And no significant difference in serum chromium concentration between the patients and the control group.Regarding the polymorphisms of haptoglobin, the study did not find a statistically significant relationship between these genetic variations and the levels of iron, copper, and chromium in any of the participant groups. However, the concentrations of zinc differed between the three types of haptoglobin.

References

- Edwards, O., Burris, A., Lua, J., Wilkie, D. J., Ezenwa, M. O., &Doré, S. (2022). Influence of Haptoglobin Polymorphism on Stroke in Sickle Cell Disease Patients. Genes, 13(1), 144.

- Hardouin, G., Magrin, E., Corsia, A., Cavazzana, M., Miccio, A., &Semeraro, M. (2023). Sickle Cell Disease: From Genetics to Curative Approaches. Annual Review of Genomics and Human Genetics, 24, 255-275.

- Almosfer, M. A. (2022). HPLC and Capillary Electrophoreses Techniques for the Screening of Sickle Cell Anemia Disease Among Adults in Saudi Arabia (Doctoral dissertation, Alfaisal University (Saudi Arabia)).

- Abutaleb, A., Kottilil, S., & Rosenthal, E. (2023). Hepatitis C Virus. In Viral Infections of Humans: Epidemiology and Control (pp. 1-28). New York, NY: Springer US.

- Yağanoğlu, M. (2022). Hepatitis C virus data analysis and prediction using machine learning. Data & Knowledge Engineering, 142, 102087.

- Yang, T. H., Chan, C., Yang, P. J., Huang, Y. H., & Lee, M. H. (2023). Genetic Susceptibility to Hepatocellular Carcinoma in Patients with Chronic Hepatitis Virus Infection. Viruses, 15(2), 559.

- Tam, V., Patel, N., Turcotte, M., Bossé, Y., Paré, G., &Meyre, D. (2019). Benefits and limitations of genome-wide association studies. Nature Reviews Genetics, 20(8), 467-484.

- Gupta, S., Ahern, K., Nakhl, F., & Forte, F. (2011). Clinical usefulness of haptoglobin levels to evaluate hemolysis in recently transfused patients. Advances in Hematology, 2011.

- Ballas, S. K. (2002). Sickle cell anaemia: progress in pathogenesis and treatment. Drugs, 62, 1143- 1172.

- Kasvosve, I., Speeckaert, M. M., Speeckaert, R., Masukume, G., & Delanghe, J. R. (2010). Haptoglobin polymorphism and infection. Advances in clinical chemistry, 50, 23-46.

- Olatunya, O. S., Albuquerque, D. M., Santos, M. N. N., Kayode, T. S., Adekile, A., & Costa, F. F. (2020). Haptoglobin gene polymorphism in patients with sickle cell anemia: findings from a Nigerian cohort study. The Application of Clinical Genetics, 107-114.

- Ruiz, M. A., Shah, B. N., Ren, G., Hussain, F., Njoku, F., Machado, R. F., ... &Saraf, S. L. (2022). Haptoglobin 1 allele predicts higher serum haptoglobin concentration and lower multiorgan failure risk in sickle cell disease. Blood Advances, 6(24), 6242-6248.

- Van Vlierberghe, H., Delanghe, J. R., De Bie, S., Praet, M., De Paepe, A., Messiaen, L., ... &Leroux- Roels, G. (2001). Association between Cys282Tyr missense mutation and haptoglobin phenotype polymorphism in patients with chronic hepatitis C. European journal of gastroenterology &hepatology, 13(9), 1077-1081.

- Awad, N. A. (2013). estimation of some important essintial elements levels se, zn and fe in the sickle cell anemia patiants serum in basrah. University of Thi-Qar Journal of Science, 3(4), 84-86.

- Shi, Y., Zou, Y., Shen, Z., Xiong, Y., Zhang, W., Liu, C., & Chen, S. (2020). Trace elements, PPARs, and metabolic syndrome. International journal of molecular sciences, 21(7), 2612.

- Himoto, T., & Masaki, T. (2020). Current trends of essential trace elements in patients with chronic liver diseases. Nutrients, 12(7), 2084.

- Franzago, M., Santurbano, D., Vitacolonna, E., &Stuppia, L. (2020). Genes and diet in the prevention of chronic diseases in future generations. International journal of molecular sciences, 21(7), 2633. 18- Kazi, T. G., Afridi, H. I., Kazi, N., Jamali, M. K., Arain, M. B., Jalbani, N., &Kandhro, G. A. (2008). Copper, chromium, manganese, iron, nickel, and zinc levels in biological samples of diabetes mellitus patients. Biological trace element research, 122, 1-18.

- KengneFotsing, C. B., Pieme, C. A., BiapaNya, P. C., Chedjou, J. P., Dabou, S., Nguemeni, C., ... &Gatsing, D. (2022). Relation between haptoglobin polymorphism and oxidative stress status, lipid profile, and cardiovascular risk in sickle cell anemia patients. Health Science Reports, 5(1), e465. 20- Adekile, A. D., &Haider, M. Z. (2010). Haptoglobin gene polymorphisms in sickle cell disease patients with different βS-globin gene haplotypes. Medical Principles and Practice, 19(6), 447-450.

- Ibrahim, N., Baleela, R., Elbashir, M. I., Ahmed, H. M., Elkhider, I., &Elagib, A. A. (2012). Association of Hp 1-1 with liver disorders among Sudanese patients. Am J SciInd Res [Internet], 3(6), 403-5.

- Tebbi, C. K. (2022). Sickle Cell Disease, a Review. Hemato, 3(2), 341-366.

- Vona, R., Sposi, N. M., Mattia, L., Gambardella, L., Straface, E., &Pietraforte, D. (2021). Sickle cell disease: role of oxidative stress and antioxidant therapy. Antioxidants, 10(2), 296.

- Sumaily, K. M. (2022). The roles and pathogenesis mechanisms of a number of micronutrients in the prevention and/or treatment of chronic hepatitis, COVID-19 and type-2 diabetes mellitus. Nutrients, 14(13), 2632.

- Antwi-Boasiako, C., Dankwah, G. B., Aryee, R., Hayfron-Benjamin, C., Doku, A., N’guessan, B. B., ... & Campbell, A. D. (2019). Serum iron levels and copper-to-zinc ratio in sickle cell disease. Medicina, 55(5), 180.

- Mohanty, P., Jena, R. K., &Sethy, S. (2017). Variability of iron load in patients of sickle cell anaemia (HbSS): a study from Eastern India. Journal of clinical and diagnostic research: JCDR, 11(3), EC19.

- Dos Santos, T. E. D. J., De Sousa, G. F., Barbosa, M. C., &Gonçalves, R. P. (2012). The role of iron overload on oxidative stress in sickle cell anemia. Biomarkers in Medicine, 6(6), 813-819.

- Schmidt, S. M. (2020). The role of iron in viral infections. Frontiers in Bioscience-Landmark, 25(5), 893-911.

-Zou, D. M., & Sun, W. L. (2017). Relationship between hepatitis C virus infection and iron overload. Chinese Medical Journal, 130(07), 866-871.

- Milic, S., Mikolasevic, I., Orlic, L., Devcic, E., Starcevic-Cizmarevic, N., Stimac, D., ... &Ristic, S. (2016). The role of iron and iron overload in chronic liver disease. Medical science monitor: international medical journal of experimental and clinical research, 22, 2144.

- Lange, C. M., Kutalik, Z., Morikawa, K., Bibert, S., Cerny, A., Dollenmaier, G., ... & Swiss Hepatitis C Cohort Study Group. (2012). Serum ferritin levels are associated with a distinct phenotype of chronic hepatitis C poorly responding to pegylated interferon‐alpha and ribavirin therapy. Hepatology, 55(4), 1038-1047.

- Jabbar, E. A. K., Maktoof, A. A., &Jouda, J. (2020). Evaluation of Metal levels and Physiological parameters in Sickle cell anemia and their comparison with Iron deficiency anemia. Research Journal of Pharmacy and Technology, 13(10), 4655-4660.

- Hassan, R. E., &Kaddam, L. A. (2021). Assessment of Salivary Zink Level and its impact on Periodontal Health among Children and Adolescence with Sickle Cell Anemia.

- A Aldabagh, M., I Kader, S., & M Ali, N. (2011). • Serum levels of copper, zinc, iron and magnesium in Iraqis patient with chronic hepatitis C. Kerbala Journal of Medicine, 4(10), 1146-1150.

- Aslam, N., Iqbal, M. S., Hussain, S. M., Rizwan, M., Naseer, Q. U., Afzal, M., ... &Batool, F. (2019). Effects of chelating agents on heavy metals in Hepatitis C Virus (HCV) patients. Mathematical Biosciences and Engineering, 16(3), 1138-1149.

- Anber, N. H., El-Ghannam, M. Z., El-Kheshen, G. A., & Bialy, M. I. (2016). Evaluation of serum zinc level in Egyptian patients with hepatitis C-associated cirrhosis. Journal of Pharmaceutical and Biomedical Sciences, 6(2).

- Guo, C. H., Chen, P. C., &Ko, W. S. (2013). Status of essential trace minerals and oxidative stress in viral hepatitis C patients with nonalcoholic fatty liver disease. International Journal of Medical Sciences, 10(6), 730.

- Jomova, K., Makova, M., Alomar, S. Y., Alwasel, S. H., Nepovimova, E., Kuca, K., ... &Valko, M. (2022). Essential metals in health and disease. Chemico-biological interactions, 110173.

- Nnodim, J. K., Samuel, M., Dioka, C. E., Onah, C. E., Ihim, A., &Atuegbu, C. (2014). Trace elements deficiency in patients with homozygous sickle cell disease. British Journal of Medicine and Medical Research, 4(21), 3878.

- Garba, N., Ifeanyichukwu, O. M., Amilo, G. I., &Audu, I. (2016). Evaluation of trace elements in adult sickle cell anaemia patients in Zaria, North Western Nigeria. J Blood DisordTransfus, 7(2), 1000347.

- Jena, A. B., Samal, R. R., Bhol, N. K., &Duttaroy, A. K. (2023). Cellular Red-Ox system in health and disease: The latest update. Biomedicine & Pharmacotherapy, 162, 114606.

- Gaetke, L. M., & Chow, C. K. (2003). Copper toxicity, oxidative stress, and antioxidant nutrients. Toxicology, 189(1-2), 147-163.

- Emokpae, M. A., &Fatimehin, E. B. (2020). Copper-to-zinc ratio as an inflammatory marker in patients with sickle cell disease. Sci, 2(4), 89.

- Osredkar, J., &Sustar, N. (2011). Copper and zinc, biological role and significance of copper/zinc imbalance. J Clinic Toxicol S, 3(2161), 0495.

- Tamai, Y., Iwasa, M., Eguchi, A., Shigefuku, R., Sugimoto, K., Hasegawa, H., & Takei, Y. (2020). Serum copper, zinc and metallothionein serve as potential biomarkers for hepatocellular carcinoma. PLoS One, 15(8), e0237370.

- Hatano, R., Ebara, M., Fukuda, H., Yoshikawa, M., Sugiura, N., Kondo, F., ... &Saisho, H. (2000). Accumulation of copper in the liver and hepatic injury in chronic hepatitis C. Journal of gastroenterology and hepatology, 15(7), 786-791.

- Gupta, S., Read, S. A., Shackel, N. A., Hebbard, L., George, J., &Ahlenstiel, G. (2019). The role of micronutrients in the infection and subsequent response to hepatitis C virus. Cells, 8(6), 603. 48- Ramdas, M., Manpreet, K., Afroze, A., Malviya, S. N., &Dangi, C. B. S. (2014). Trace elements ratio in patients of haemoglobinopathies. Int J CurrMicrobio App Sci, 3(6), 81-92.

- Cahill, L. E., &Rimm, E. B. (2015). Diet–Gene Interactions: Haptoglobin Genotype and Nutrient Status. Preventive Nutrition: The Comprehensive Guide for Health Professionals, 115-129. 50- Na, N., Delanghe, J. R., Taes, Y. E., Torck, M., Baeyens, W. R., &Ouyang, J. (2006). Serum vitamin C concentration is influenced by haptoglobin polymorphism and iron status in Chinese. Clinicachimicaacta, 365(1-2), 319-324.

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Published

2023-12-03