Molecular Study of Co-Infection between Giardia Lamblia and Helicobacter Pylori in Non- Symptomatic Children in Thi-Qar Province,Iraq
DOI:
https://doi.org/10.32792/jeps.v10i1.40Keywords:
Giardia lamblia, Helicobacter pylori, co-infection, PCRAbstract
The current study was carried out in Thi-Qar Province which included a collection of stool samples fromnon-symptoms children. The aim of the study was to detect of co-infection of Giardia. lamblia with
Helicobacter pylori in non-symptoms children. Atotal 96 stool samples taken children were under five
years of both sex and examined by PCR for detection of G. lamblia. The results showed that the percentage
of positive samples was 32 positive and 64 negative. The most infection was in females more than males.
The highest infection with G. lamblia according to PCR was in rural more than in urban population. The
highest infection rate with G. lamblia in the age group (1-12 months) , Patients and the lowest age group
(37-48 months) .A total of 96 samples were examined by PCR for diagnosis of G. lamblia also to
detection of H. pylori . The results showed 32 positive samples with G. lamblia and only 14 positive
samples with H. pylori. The number of samples that have co-infection between G. lamblia with H. pylori
is 13. The BLAST results for DNA sequencing showed that isolation for G. lamblia different with the
isolates recorded at the National Center for Biotechnology (NCBI); therefore, these isolates were
registered in NCBI as new isolates under the GenBank accession numbers, that including two isolates per
microorganism. G. lamblia, isolate IQ No.1 (MN096738) and G. lamblia co-infection with H. pylori
isolate IQ No.2 (MN096739). The accessions number for isolates of H. pylori are, seq1(MN115555) and
H. pylori co-infection with G. lamblia isolates seq2 (MN115556).
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