Determination of Bioactive Compounds and Antibacterial of The Brown algae Sargassum sp.

Abstract

Objective of this study was to determine of bioactive compounds and antibacterial activity of  Sargassum Sp extract. Pathogenic bacteria, including Pseudomonas aerogenosa, Escherichia coli, Salmonella SP., Bacillus Cereus, Staphyloylococcus aureus,  and Streptococcus mutans  , as the active chemical compounds were extracted from this algae using solvents (cold water Ethanol  and methanol alcohol) with qualitative detection of secondary metabolites of the aqueous extract of this algae, which indicated the presence of phytochemicals analysis such as Amino acid, Alkaloids, Steroids, Phenols, Flavonoids, Glycosides, Terpenoids, Tan inns, Saponins and Carotenoids .  The chemical compounds were determined using the gas chromatography mass spectroscopy (Gc-mass) technique of the methanolic extract of  sargassum These compounds were represented Muramic acid, Isopropyl Alcohol, Glycerin, Loliolide, Tetradecanoic acid, Pentadecanoic acid, n-Hexadecenoic acid,  , Phytol,  Oleic acid, Octadecanoic acid,   Erucic acid,  Ergosta-5,24(28)-dien-3-ol,(3.beta.)- and   Stigmasta-5-24(28)-dien-3-ol,( 3.beta)- The aqueous extract of this algae did not show any bioactivity against all bacterial species under study. It was noticed that the methanolic extract was more efficient in its effect on the bacterial species, as the rates of inhibition zones for this extract were (16, 18.66, 16.66, 19, 19.33 and 22.66) mm for each of P. aerogenosa, E. coli, Salmonella Sp., B. cereus., Staph. aureus and S. mutans, respectively.