Identification immunohistological changes in spleen in meals and females with endocrine glands disorder

Abstract

Abstract

            Endocrine disorders are agroup of condition resulting from problems in part of the endocrine system.These disorders occur when the endocrine system produces too much or too little of ahormone,or the body does not respond in the correct way to the hormone.This study amis to find out the effect of endocrine disorders on spleen of male and female laboratory rats.Twenty− four rats were divide in to three group.The first group was a control group eight rats(4 males and 4 females): received a normal saline solution for 21 days. The second group (8 males rats): rat were given 0.6 mg ̸ kg metoclopramide by intraperitoneal injection for hyperprolactinemia induction for 21 days. The third group (8 females rats):rat were given 0.6 mg ̸ kg metoclopramid by intraperitoneal injection for hyperprolactinemia induction for 21 days.  After the end of experimental, 21 days some blod parameters were measured and the level of the hormone prolactin was measured.The spleen were removed,and their after their removal for the three groups.The tissue was sectioned and stained with hematoxylin,eosin and masson stain..The study also showed a significant increase (p<0.05) in the concentration of the hormone prolactin for the male and femals group compared to the control group.Histological examination of the spleen showed a noticeable change Note the white pulp increased  area covered about 50% of the spleen area compared with the red pulp  area  ,The secondary and lymph follicle germi center was observed, when compared it with the control ,we notice a smaller white pulp area, and the secondary follicle, the active follice, was not observed.