The effect of metabolic products of Enterococcus faecalis strains on the cell toxicity
DOI:
https://doi.org/10.32792/jeps.v10i1.47Keywords:
LAB, Cytotoxicity, VERO cells, pathogen, hemolycinAbstract
Enterococcus faecalis strains are common commensal of the intestines of humans and other animals. Production of different metabolic substance from Enterococci has highest effect on the microorganism and cytotoxicity. The present study highlights the effect of metabolic productions on the cell toxicity by used VERO cells. The results also showed that the metabolic productions of E. faecalis A1 and E. faecalis A2 were toxic untile dilution factor 12 and had cytoxicity effect on the VERO cells, While the E. faecalis A3 was less toxicity at dilution 1/8References
Ahmed, W., Payyappat, S., Cassidy, M., & Besley, C. (2019). Enhanced insights from human and animal
host-associated molecular marker genes in a freshwater lake receiving wet weather overflows, (April), 1–
https://doi.org/10.1038/s41598-019-48682-4
Alvarez-Cisneros, Y., & Espuñes, T. (2011). Enterocins: Bacteriocins with applications in the food
industry. Formatex.Info, 1330–1341. http://www.formatex.info/microbiology3/book/1330-1341.pdf
Bang, N., Jensen, S., Melchiorsen, C. R. I. X., Jokumsen, K. V., & Villadsen, J. (2001). Metabolic
Behavior of Lactococcus lactis MG1363 in Microaerobic Continuous Cultivation at a Low Dilution Rate,
(6), 2677–2682. https://doi.org/10.1128/AEM.67.6.2677
Ben Said, L., Klibi, N., Lozano, C., Dziri, R., Ben Slama, K., Boudabous, A., & Torres, C. (2015).
Diversity of enterococcal species and characterization of high-level aminoglycoside resistant enterococci
of samples of wastewater and surface water in Tunisia. Science of The Total Environment, 530–531, 11–
https://doi.org/10.1016/j.scitotenv.2015.05.091
Coburn, P. S., & Gilmore, M. S. (2003). The Enterococcus faecalis cytolysin: a novel toxin active against
eukaryotic and prokaryotic cells. Cellular Microbiology, 5(10), 661–669. Retrieved from
http://www.ncbi.nlm.nih.gov/pubmed/12969372
Goto, D. K., & Yan, T. (2011). Effects of Land Uses on Fecal Indicator Bacteria in the Water and Soil of
a Tropical Watershed. Microbes and Environments, 26(3), 254–260.
https://doi.org/10.1264/jsme2.ME11115
Hayashi, H., Takahashi, R., Nishi, T., Sakamoto, M., & Benno, Y. (2005). Molecular analysis of jejunal,
ileal, caecal and rectosigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal
restriction fragment length polymorphism. Journal of Medical Microbiology, 54(11), 1093–1101.
https://doi.org/10.1099/jmm.0.45935-0
Henning, C., Gautam, D., & Muriana, P. (2015). Identification of Multiple Bacteriocins in Enterococcus
spp. Using an Enterococcus-Specific Bacteriocin PCR Array. Microorganisms, 3(1), 1–16.
https://doi.org/10.3390/microorganisms3010001
Huang, E., Zhang, L., Chung, Y. K., Zheng, Z., & Yousef, A. E. (2013). Characterization and application
of enterocin RM6, a bacteriocin from enterococcus faecalis. BioMed Research International, 2013, 1–7.
https://doi.org/10.1155/2013/206917
Izquierdo, E., Wagner, C., Marchioni, E., Aoude-werner, D., & Ennahar, S. (2009). Enterocin 96 , a Novel
Class II Bacteriocin Produced by Enterococcus faecalis WHE 96 , Isolated from Munster Cheese ????,
(13), 4273–4276. https://doi.org/10.1128/AEM.02772-08
Jamet, E., Akary, E., Poisson, M. A., Chamba, J. F., Bertrand, X., & Serror, P. (2012). Prevalence and
characterization of antibiotic resistant Enterococcus faecalis in French cheeses. Food Microbiology,
(2), 191–198. https://doi.org/10.1016/j.fm.2012.03.009
Kim, M. H., Kim, S. Y., Son, J. H., Kim, S. Il, Lee, H., Kim, S., … Lee, J. C. (2019). Production of
Membrane Vesicles by Enterococcus faecium Cultured With or Without Subinhibitory Concentrations of
Antibiotics and Their Pathological Effects on Epithelial Cells, 9(August), 1–11.
https://doi.org/10.3389/fcimb.2019.00295
Klein, G. (2003). Taxonomy, ecology and antibiotic resistance of enterococci from food and the gastrointestinal
tract. International Journal of Food Microbiology, 88(2–3), 123–131.
https://doi.org/10.1016/S0168-1605(03)00175-2
Layton, B. a., Walters, S. P., Lam, L. H., & Boehm, a. B. (2010). Enterococcus species distribution among
human and animal hosts using multiplex PCR. Journal of Applied Microbiology, 109(2), 539–547.
https://doi.org/10.1111/j.1365-2672.2010.04675.x
Martín-Platero, A. M., Valdivia, E., Ruíz-Rodríguez, M., Soler, J. J., Martín-Vivaldi, M., Maqueda, M.,
& Martínez-Bueno, M. (2006). Characterization of antimicrobial substances produced by Enterococcus
faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops). Applied and
Environmental Microbiology, 72(6), 4245–4249. https://doi.org/10.1128/AEM.02940-05
Mehmeti, I., Jo, M., Fergestad, E. M., Mathiesen, G., Nes, I. F., & Holo, H. (2011). Transcriptome ,
Proteome , and Metabolite Analyses of a Lactate Dehydrogenase-Negative Mutant of Enterococcus
faecalis V583 ???? †, 77(7), 2406–2413. https://doi.org/10.1128/AEM.02485-10
Nishie, M., Nagao, J.-I., & Sonomoto, K. (2012). Antibacterial peptides “bacteriocins”: an overview of
their diverse characteristics and applications. Biocontrol Science, 17(1), 1–16. Retrieved from
http://www.ncbi.nlm.nih.gov/pubmed/22451427
Tanaka, K., Watabe, T., Kato, K., Tsukimi, T., Sato, M. P., & Odamaki, T. (2019). crossm Draft Genome
Sequences of Enterococcus faecalis Strains, (October), 17–19.
Zi, P., Gnat, S., & Nowakiewicz, A. (2017). Characterization of Multidrug Resistant E . faecalis Strains
from Pigs of Local Origin by ADSRRS-Fingerprinting and MALDI -TOF MS ; Evaluation of the
Compatibility of Methods Employed for Multidrug Resistance Analysis, 1–19.
https://doi.org/10.1371/journal.pone.0171160
Zommiti, M., Cambronel, M., Maillot, O., & Barreau, M. (2018). Evaluation of Probiotic Properties and
Safety of Enterococcus faecium Isolated From Artisanal Tunisian Meat “ Dried Ossban ,” 9(August), 1–
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