A Molecular Detection for Some Bacterial Resistance Genes Escherichia Coli Isolated from Fresh Red Meat in City of Al- Nasiriyah
Keywords:
Red meat contamination, Escherichia coli, Sensitivity test, Resistance genesAbstract
The present study was conducted to detect the contaminated microbial pathogens of fresh red meat indifferent places of the al-butcher shops, where 100 samples and swabs were collected from different places
and tools from meat shops in different areas in Nasiriyah for the period from September 2018 to March
2019. The highest isolation rate for Escherichia coli isolates was different according to the type of sample
taken (31.53% meat samples, 21.42% the ground, 21.33% cutting boards, 19.1% cutting tools). 15 isolates
were selected for the sensitivity test for eight antibiotics, to detect resistance genes, sul1 and tet (B) The
isolates were highly resistant to Ampicillin, Vancomicin, Amoxicillin at a rate of 100%, they were very
sensitive to antimicrobial antiperspirants such as Trimethoprim, Norfloxacin, Chloramphenicol at a rate
of 100%, The sensitivity of Tetracycline and Gentamycin was 86.7% and 80% respectively. The results
of the electrical relay were shown after DNA extraction using Kit and amplified the genes using a PCR.
Specialized gene primers were used for each gene, the presence of the resistant gene sul1 in the isolates
of E. coli was 80%, and the tet (B) was 87.3%. Some isolates were record as new strains in the NCBI
gene bank database.
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