Molecular Characterization and Antibiotic sensitivity of Staphylococcus sp. Isolated from Animal Skin wounds

Abstract

A wide variety of commensal microorganisms reside on the skin, many of which secrete proteolytic enzymes that might have detrimental effects following an injury. These bacteria are considered opportunistic pathogens. The objective of the study is to isolate Staphylococcus sp. from animal skin and conduct molecular characterization of the recovered strains in order to examine their enzymatic production capabilities. The objective is to assess the antibiotic susceptibility of Staphylococcus sp. and determine the ideal conditions for bacterial development. A total of 25 swab samples were obtained from animal skin wounds in AL-Shatrah city. Each sample was cultured on nutrient agar, blood agar, and Mannitol salt agar (with a 7.5% NaCl concentration) as a specialized medium. All isolates underwent molecular diagnosis using the PCR technique. Additionally, 10 isolates were selected for sequencing using the Sanger dideoxy 16S rRNA sequencing method. Enzyme production properties were analyzed, and the sensitivity of bacteria to six antibiotics was studied. Additionally, the researchers investigated the ideal circumstances for bacterial proliferation. Analysis of 25 swab samples taken from animal skin wounds yielded a total of 18 isolates. Based on biochemical tests, the majority of these isolates were identified as Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus epidermidis, and Staphylococcus borealis. Staphylococcus warneri and Staphylococcus epidermidis exhibited sensitivity to Novobiocin, with inhibition zones of 22mm and 21mm respectively. However, all other isolates that were examined showed resistance to novobiocin.