Effect of fertilization by Cladophora sp. on biochemical characteristics of Vigna radiate & Sesamum indicum plants

  • Basim Y. Alkhafaji
  • Roaa J. Elkheralla
  • Haider R. Malih
Keywords: Cladophora sp, Vigna radiata L,, Sesamum indicum


An experiment was done in pots at the garden of biology department, College of Science, University of Thi-Qar during September until November 2018. The experiment aimed to Know the chemical content of Cladophora sp. and it use in soil amendment as organic fertilizer, as well as its effect on some biochemical characteristics (chlorophyll content, protein, carbohydrate and ash).
The experiment contained the following treatments: control (c) no adding algae, (1%), (2%) (3%) wich adding Cladophora sp. as (300, 600, and 900) gm / 30 kg soil respectively. All Cladophora sp. treatments showed significant increase in all biochemical characters compared with the control. (3%) treatment gave significant increase as compared to other treatments in (chlorophyll content; protein content; shoot, carbohydrate content and ash content) in both plants.
Vigna radiate showed increasing percentage in chlorophyll content, protien, carbohydrates and ash (11.2% ,21.7%, 27.1% and 5.5%) respectively in T3(3%).As for Sesamum indicum, it also showed significant differences in biochemical characteristics in all the treatments and the highest values in (chlorophyll content, protien and carbohydrates) was (98.2%, 20.9% ,18.8% and 5.3%) respectively in T3(3%)


• A.O.A.C. (1990): Official Methods of Analysis. Association of Official Analytical Chemists.

• Abd El Moniem, E. A. and Abd-Allah A. S. E. (2008). Effect of green algae cells extract as foliar spray on vegetative growth, yield and berries quality of superior grapevines. J. Amer. Eur. Agric. and Environ. Sci., 4 (4), 427-433.

• Al-Gosaibi, A. M. (1994). Use of algae as a soil conditioner for improvement of sandy soils in Al-Ahasa, Saudi Arabia. J. Agric. Sci. Mansoura Univ., 19(5): 1877-1883.antioxidant activity for multiple benefits. Virginia Vegetable, Small Fruit and Special

• Arnon, D. I. (1949): Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24,1-15.

• Bloemberg GV, Wijfjes AHM, Lamers GEM, Stuurman N &Lugtenberg BJJ (2000) Simultaneous imaging of Pseudomonas fluorescens WCS365 populations expressing three different auto-fluorescent proteins in the rhizosphere: new perspectives forstudying microbial communities. Mol. Plant-Microbe Interact.13: 1170–1176

Crops., 2(6), 1 – 3.

• DuBois, M., Gilles, K., Hamilton, J., Rebers, P., and Smith, F. (1956): Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3),350–356.

• Eid, Mohammed bin Abdul Rahman. Al-Jarwani, Mohamed Mohamed. Hamail, Ali Fathi. Study of the Effect of Fertilization on Phosphate Fertilizers on the Accumulation of Cadmium in Soil and in the Vegetable Parts of Some Vegetable Crops, Faculty of Science (2002).

• El-Fouly, M.M., F.E. Abdalla and M.M. Shaaban, 1992. Multipurpose large scale production of microalgae biomass in Egypt Proc. 1st Egyptian Etalian Symptoms on Biotechnology, Assiut, Egypt (Nov., 21-23), pp: 305–14.

• Goel, A.K., R.D.S. Laura, G. Pathak, G. Anuradha and A. Goel, 1999. Use of bio-fertilizers: potential, constraints and future strategies review. Int. J. Trop. Agric., 17: 1 18.

• He, Z. L., Alva, A. K., Yan, P. Li, Y. C., Calverat, D. V., Stoffella, P. J., Banks, D.J. Nitrogen mineralization and transformation from composts and biosolids during field incubation in sandy soil.2000; Soil Sci.165,161-169. -3.

• Hegde, D.M., B.S. Dwivedi and S.N.S. Babu, 1999. Bio-fertilizers for cereal production in India- A review. Ind. J. Agric. Sci., 69: 73–83. Mandimba, G.R., G.D. Okomba and Pandzou, 1998. Nodulated Legumes as green manure: an alternative source of nitrogen for non-fixing and poor fixing crops. Int. J. Trop. Agric., 16: 131–45. Boussiba, S., 1987. Anabaena azollae as nitrogen bio-fertilizer. In: Barking, S.T. (ed.), Algal Biotechnology, pp: 169–78. Elsevier Applied Science Publishers, The Netherlands.

• Hussein, A.H.A. Impact of sewage slude organic manure on soil properties. Growth, yield and nutrient cantents of cucumber crop. J. of applied sciences.2009;9(8): 1401-1411. J. Plant Physiology, 115, 433-437.

• Jackson, M. LSoil chemical analysis Ed. prentideHall.Engle wood cliffas. Newjersey, USA. .(1958).

• Mahmoud, M.S., 2001. Nutritional status and growth of maize plants as affected by green microalgae as soil additives. J. Biol. Sci., 1: 475–9. Shaaban, M. M. (2001). Green microalgae water extract as foliar feeding to wheat plants. Pakistan Journal of Biological Sciences 4(6): 628-632

• Nelson, D. W. and Sommers, L. E. Organic Matter, In: “Methods of Soil Analysis, Part Chemical and Microbiological Properties”. (Ed.): Page, A. L., American Society of Agronomy, Madison, WI. 1982; PP. 574-579.

• Nelson, W. R.; Van Staden, J. (1984). The effect of seaweed concentrates on wheat Culms.

• O, Dell, C. (2003). Natural plant hormones are biostimulants helping plants develop plant

• PiJuam. Josef, Aida valls, Ana pasullo and Marta shuhmadir. Evaluation the impact of sewage sludge application on agricultural soils. Health ,305 defebreru. (2010)

• Prescott, G.W. (1962): algae of the western great lakes area. 2nd ed w. c brown. co. dubuque, lowa pp 997.

• Rhoades, J. D. Salinity: Electrical Conductivity and Total Dissolved Solids, In: “Methods of Soil Analysis, Part III, Chemical Methods”. (Ed.): Sparks, D. L., American Society of Agronomy, Madison, WI. 1996.; PP. 417-436.