Evaluation of Bioactivity of Maximal Inhibitory Concentration of Alkaloids Isolated from Mentha spicata Leaves Against Some Pathogenic Fungi

  • Kawkab Ali Hussain Department of Chemistry – College of Education for Pure Sciences - University of Basrah Basrah – Iraq
  • Abbas Dawwas Matter Al-Maliki Department of Chemistry – College of Education for Pure Sciences - University of Basrah Basrah – Iraq
Keywords: Mentha spicata leaves, Alkaloids, Maximal inhibitory concentration, Bioactivity, pathogenic fungi.


Alkaloids were isolated and identified from Mentha spicata leaves. Qualitative analysis, thin layer chromatography(TLC), functional groups test and infra-red spectrum were carried out for identification of these alkaloids. TLC results showed presence of two alkaloidic compounds in alkaloids extract. The Bioactivity and biochemical effect of alkaloids, was investigated towards four pathogenic fungi. The maximal inhibitory concentration was measured to be (250 mg/ml) and the Bioactivity of this concentration was recorded against pathogenic fungi represented by Aspergillus fumigatus, Candida albicans, penicillum crysosporm and Aspergillus niger with inhibition zone diameters equal to (23, 15, 15 and 11 mm) respectively. Therefore, the alkaloidic compounds isolated can be used as an herbal theuraputic substituent to the different diseases caused by these fungi but this work demands further clinical and pharmaceutical studies.


-Pramila, D. M.; Xavier, R.; Marimuthu, K.; Kathiresah, S.; Khoo, M. L.; Senthilkumar, M.; Sathya, K and Sreeramanan, S. (2012). Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (mentha piperita: Lamiaceae). J. Medicinal plants Res.; 6(2):331-335.

-Padmini, E; Valarmathi, A. and Usha Rani, M. (2010). Comparative analysis of chemicalcomposition and antibacterial activities of Mentha spicata and Camellia sinensis. Asian J. Exp.Biol. Sci, 1(4): 772- 781.

-Kurian, J. C. (2012). Ethno-medicinal plants of India, Thailand and Vietnam. J. Biodiversity, 3(1):61- 75.

-Parrota, J. A. (2001). Healing Plants of Peninsular India. CABI Publishing, NewYork, USA.

-Padmini, E; Prema, K.; Vijaya, G. B. and Usha R. M. (2008). Comparative study on composition and antioxidant properties of mint and tea extract. Int. J. Food. Sci. Tech.43:1887-1895.

-Mckay, D. L. and Blumberg. J. B. (2006). A review of the bioactivity and health benefits of peppermint tea (Mentha piperita L). Phytother. Res., 20:619-633.

-Naseem, U.; Muhammad, K.; Muhammad, U. A.; Hamid, H. A.; Farhat, A. K.; Sahibzada, M. U.K.; Saleem, U.; Umberin, N.; Javid, H. and Asif, K. (2011). Comparision of Phytochemical constituents and antimicrobial activities of Mentha spicata from four northern districts of Khyber Pakhtunkhwa. J. Appl. Pharama. Scie., 1: 72-76.

-Sweetie, R. K.; Ramesh, C. and Arun, S. (2007). Antioxidant potential of mint (Mentha spicata L) in radiation – processed lamb meat. Food chemistry, 100(2):451- 458.

-Rasooli, I. (2008). Dental biofilm preventation by Mentha spicata and Eucalyptus camaldulensis essential oils. Int. J. Infect. Dis., 12(1):167-174.

-Al-Maliki, A.D.M. (2011). Isolationand Identification of an alkaloidic compound from Coriandrum sativum seeds and study of its medicinal activity against pathogenic bacteria of urinary tracts. J. Basrah Res.(Sciences).37(2):119- 127.

-Harborne, J. and Baxter, H. (1993). Phytochemical Dictionary. Taylor&Francis Publishers, London, UK.

. ed, chapman and Hall, London, UKndmethods. 2 arbores, J. (1984). PhytochemicalH-12

, Chapman and Hall, ed. stmethods. 1 Harborne, J. (1974). Phytochemical-13NewYork, USA.

- Harbore, J. (1984). Phytochemical methods. 2nd.ed, chapman and Hall, London, UK.

-Harborne, J. (1974). Phytochemical methods. 1st. ed, Chapman and Hall, NewYork, USA.

-Bruneton, J. (1995). Pharmacognosy phytochemistry medicinal plants. Lavoisier Pubs. Paris, France.

-Cowan, M. (1999). Plant products as antimicrobial agents. Clin. Microb. Rev., 12:564-582.

-Feeny, P. (1998). Inhibitory effect of oak leaf tannins on the hydrolysis of proteins by trypsin, J. Phytochem., 8: 2116-2121.

- Castillo, J.; Gonzalez, V.; Jaime, A. H; Martinez, G.; Linares, E.; Bye, R. and Romero, I. (2009). Anti-helicobacter pylori activity of plants used in mexican traditional medicine for gastrointestinal disoreders. J. Ethnopharmacol., 122: 402- 405.

- Britto, J.; Steena, R. S. and Mary, S. R. (2011). Phytochemical analysis of eight medicinal plants of Lamiaceae. J. Res. Plant Sci., 1(1): 1-6.

-Akinmo, A. C.; Ibukun, E.O.; Afor, E.; Obuotor, E. M. and Farombi, E. O. (2007). Phytochemical constituents and antioxidant activity of extracts from leaves of Ocimum gratissimum. Sci. Res.Essay. 2: 163-166.

-Nurdan, S. and Aysel, U. (2007), Antimicrobial activities and usage in folkloric medicine of some Lamiaceae species growing in Mugla, Turkey. Eur. Asia J. Bio. Sci.,4:28-37.

-Gabriela, C. (2009). Plant Extracts: TLC analysis. Encyclopedia of. Sussex, UK Analytical Instruments Ltd., East Sirius ed., rd3Chromatography.

-Gao, X. X.; Liang, C. Q. and Chen, X. Y. (2007). Preliminary study on TLC fingerprint of radix (Polygoni multilory) from different areas. Zhong Yao Cai, 30(4): 407-409.

-Silverstein, R. and Webster, F. (1997). Spectrometric identification of organic.ed., Publishers, London, UKthand Sons. Inc., 6 compounds. John Wiley

-Cosley-Smith, J. R. (1997). Coumarin in the treatment of lymphoedema and other high- protein oedemas. John Wiley and Sons, Inc., NewYork, USA, P. 348.

-Rout, S. P.; Choudhary, K. A.; Kar, D. M.; Das, L, and Jaim, A. (2009). Plants in traditional medicinal system future source of new drugs. Int. J. Pharmacy and pharmaceutical Sci,1(1): 1-23.

-Jayasurriya, H.; Nuphavan, M.; Geahlen, R.; Mclanghlin, J. and Chang, C. (1992). Emodine, a protein-kinase inhibitor from poygonum cusp. J. Nat. Prod., 55(5): 696-702.

-Collee, J.; Fraser, A.; Marimion, B. and Bimon, A. (1996). Practical Livingston. ed., Churchill thMccarteney. 5 and medical microbiology, MakieNewYork, USA.