• Fadhil , A. M. Department of Biology, College of Education for pure Science, University of Thi-Qar, Iraq.
  • Abdul Wahab, R. A Department of Biology, College of Education for pure Science, University of Thi-Qar, Iraq.
  • Khadija A.T. Department of Biology, College of Education for pure Science, University of Thi-Qar, Iraq.
Keywords: Aqueoueous extract, Gc – mass, Chlorella, Entamoeba histolytica


The objective of this study was to conduct in vivo to detect the effect of Chlorella extract on Entamoeba histolytica. and to examine its therapeutic effect in male laboratory rats type Mus musculus. These rats were dosed with a concentration of (1, 1.5 and 2) mg/ml of the aforesaid extract at a daily dose of (10, 15 and 20) days and then, the rats’ faeces were tested to observe the change in parasite numbers after dosing the extract. The results showed that the aqueoueous extract of Chlorella sp. was effective in reducing the vesicated and fed stages Entamoeba histolytica-infected rats, where the rate of killing those stages had recorded the highest percentage at dose of 2mg/ml during a period of 20 days. Moreover, qualitative detections of chemicals for secondary metabolism of aqueoueous extract of Chlorella sp. were carried out, which indicated the presence of chemically active compounds. The therapeutic efficiency of the extract was also calculated, which has recorded 62.70%.
Chemical compounds were diagnosed using the gas chromatography technique - gas chromatography mass spectrometry (Gc -mass). These compounds were represented by 1- (5-Bicyclo [2.2.1] heptyl) ethylamine, 1.4-Bis [5- [3-trifluoromethylphenyl]. 1, 2,4-oxadiazol-3-yl] benzene Pentadeoanoic, acid, Estra-1,3,5 (10) -trin-17. beta. -0l, 1-Heneicosanol, 1,2,4-Triazol, 4 - [N- (2-hydroxyethyl) -N-nitro] amino-, 9,12-Octadecadienoic acid (Z, Z), 6-octadecadienoic acid, (Z) -, 9-Oxa-bicyclo [3.3.1] Noonan-2,6-dione, cyclopentanicarboxylic acid, 2-acetyl-5-methyl-).


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