EFFECT OF AQUEOUS EXTRACT OF CHLORELLA SP. ON ENTAMOEBA HISTOLYTICA PARASITE IN VIVO

Authors

  • Fadhil , A. M. Department of Biology, College of Education for pure Science, University of Thi-Qar, Iraq.
  • Abdul Wahab, R. A Department of Biology, College of Education for pure Science, University of Thi-Qar, Iraq.
  • Khadija A.T. Department of Biology, College of Education for pure Science, University of Thi-Qar, Iraq.

DOI:

https://doi.org/10.32792/jeps.v11i1.89

Keywords:

Aqueoueous extract, Gc – mass, Chlorella, Entamoeba histolytica

Abstract

The objective of this study was to conduct in vivo to detect the effect of Chlorella extract on Entamoeba histolytica. and to examine its therapeutic effect in male laboratory rats type Mus musculus. These rats were dosed with a concentration of (1, 1.5 and 2) mg/ml of the aforesaid extract at a daily dose of (10, 15 and 20) days and then, the rats’ faeces were tested to observe the change in parasite numbers after dosing the extract. The results showed that the aqueoueous extract of Chlorella sp. was effective in reducing the vesicated and fed stages Entamoeba histolytica-infected rats, where the rate of killing those stages had recorded the highest percentage at dose of 2mg/ml during a period of 20 days. Moreover, qualitative detections of chemicals for secondary metabolism of aqueoueous extract of Chlorella sp. were carried out, which indicated the presence of chemically active compounds. The therapeutic efficiency of the extract was also calculated, which has recorded 62.70%.
Chemical compounds were diagnosed using the gas chromatography technique - gas chromatography mass spectrometry (Gc -mass). These compounds were represented by 1- (5-Bicyclo [2.2.1] heptyl) ethylamine, 1.4-Bis [5- [3-trifluoromethylphenyl]. 1, 2,4-oxadiazol-3-yl] benzene Pentadeoanoic, acid, Estra-1,3,5 (10) -trin-17. beta. -0l, 1-Heneicosanol, 1,2,4-Triazol, 4 - [N- (2-hydroxyethyl) -N-nitro] amino-, 9,12-Octadecadienoic acid (Z, Z), 6-octadecadienoic acid, (Z) -, 9-Oxa-bicyclo [3.3.1] Noonan-2,6-dione, cyclopentanicarboxylic acid, 2-acetyl-5-methyl-).

References

- Staephen J C and Horace G C (2000) Biologically active natural products: pharmaceu CRC press;

New york.

- Lincoln R, Srtupinski K and Walker J (2009) Bioactive compounds from algae.

Life Chem. Rep. 8:97-183.

- Ireland C, Copp B, Foster M, Mcdonald L, Radishy D and Swersey C (1993) Biomedical potential of

marine natural products. In: Attaway, D. and Zaborsky, O.(Eds.). Marine biotechnology,

pharmaceutical and bioactive natural products, Plenum Press, NY.143pp.

- Cannell R (1993) Algae as a source of biologically active products Pestic.Sci.,39:147-153

- El-Baroty G, Mussa M, Shallan M, Ali M, Sabah A and Shalaby E (2007) Contribution to the

aroma, Biological activities, Mineral Protein, Pigments and lipid contents of the red algae:

Asparagopsis taxiformis (Delile) trevisan. J.App.Sci.Res., 3:1825-1834.

- Lejeune M, Rybicka J and Chadee K (2009) Recent discoveries in the pathogenesis

and immune response toward Entamoeba histolytica. fut. Microb.pp.:105-118.

- D: Miceli L (2004) Distinguishing between pathogenic and non pathogenic species of Entamoeba.

Lab. Med .35:613-616.

- Moncada D, Keller K and Chadee k (2005) Entamoeba histolytica-secreted product degrades colonic

mucin oligosaccharides. Infect. Imm., 73:3790-3793.

- Marie C and Petri Jr W A (2014) Regulation of virulence of Entamoeba

histolytica. Annual review of microbiology 68: 493- 520.

- Linford A.S, Heriberto M, Kafelyn R., Hanbang Z, Singh V, Willian A and Petri JR (2009) Short

hairpin RNA. Mediated knoch down of protein expression in Entamoeba histolytica, J. Microb

:1035-1037.

- Achers JP and Mirelman D (2006) Progress in research on Entamoeba histolytica pathgenesis. J.

Microb.9, (4) :367-373.

- Ryan KJ, and Ray CG (2004) Amebiasis, Sherris Med –Microb., Mc Graw Hill, :733-738.

- Swiderski F, Dabrowski M, Rusaczone K A and Waszkiewicz B (2007) Bio active substances of garlic

and their role in dietoprophylaxis and dieto therapy Rocz panstw. Zaki. Hig.,58(1):41-60.

Journal of Education for Pure Science- University of Thi-Qar

Vol.11, No1 (June, 2021)

Website: jceps.utq.edu.iq Email: jceps@eps.utq.edu.iq

- Clark C G and Diamond L S (2002) Methods for cultivation of luminal parasitic protists of clinical

importance. Clinical Microbiology Review.15: 329–341.

- Harborne J B (1984) Methods of Plant Analysis. In Phytochemical methods :1-36. Springer, Dordrecht.

- Singh K (2012) Phytochemical Determination and Antibacterial Activity of Trichosanthes dioica Roxb

(Patal), Cucurbita maxima (pumpkin) and Abelmoschus esculentus Moench (Okra) plant seeds.Thesis -

Nationa Institute of Technology-Rourkela : 41.

- Rani D (2011) Plant Extracts with Antiamoebic properties: A Theoretical Study with reference to

Entamoeba histolytica.Int. J. of Pharm. Tech. Res, 3:1113-1117.

- De Almeida C. L.F., Faleao H. de S, Lima G. R. de athayde-Filho, P. V Barbosa-Filho, J. M. and

Batista L.M.2011. Bioacitivities from Marine Algae of the Genus Gracilaria. Int.J.Mol. Sci .2011,12, 4550-

- De Gives P. M, Arellano, M E. L, Hernandez, E.L. and Marcelino L.A(2012) Plant Extracts: A

Potential Tool for Controlling Animal Parasitic Nematodes. The Biosphere:119-130.

- Almeidi - Doria R.F. and Regitano-D Arce M (2000) Antioxixdant activity of rosemary and oregano

ethanol. Cienc. Tecnol.Aliment.20(2) :197-203.

- Cowan M.M (1999) Plant products as antimicrobial agents. Clin. Crescent, Med.J.,11:18-22.

- Kandale A, Meen A. K, Rao M. M, Panda, P, Reddy A.K.

M. G and Babu, R (2011) Marine algae: An Introduction, Food value and Medicinal uses. J. of Pharm. Res.,

:219 221.

- Naguleswaran A, Spicher M, Vonlaufen N, Ortega-Mora L, Torgerson P, Gottstein B and Hemphill A

(2006) In vitro metacestodicidal activities of genistein and other isoflavone against Echinococcus

multilocularis and Echinococcus granulosus Antimicrob.Age.Chem., 50(11):3770-3778.

- Jawad A M, Dhahir A B and Hussain a M (1985) Lactones extracted from Irsqi

composite part-1. Basrah Sci. Res. 16:5-18.

- Gilchrist CA and Petri W A (1999) Virulence factors of Entamoeba histolytica. Current opinion in

microbiology, 2(4): 433-437.

- Gopinath S, Sakthidevi G, Muthukumaraswamy S and Mohan V (2013) Gc-mass analysis of bioactive

constituents of Hypericum mysorense (Hypericaceae). J. Curr. ChemPharm.Sc., 3:6-15.

- Manilal A, Sujith S, Kiran G, Selvin J and Panikkar M (2010) Evaluation of seaweed bioactive on

common aquatic floral and faunal weeds of shrimp ponds Thalassas. Int. J. Mar. Sci. 27:47-56.

- Reardon M, Gobern S, Martinez K, Shen W, Reid T and Mcintosh M (2012) Oleic acid attenuates

trans-10, cis-12 conjugated linoleic acid-mediated inflammatory gene expression in human adipocytes.

Lipids. 47:43–51.

– Eftekhari M H, Aliasghari L F, Babaei M A and Hasanzadeh A J (2013) Effect of conjugated linoleic

acid and omega-3 fatty acid supplementation on inflammatory and oxidative stress markers in

atherosclerotic patients. ARYA atheroscler., 9(6) :311–321.

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Published

2021-06-10

Issue

Vol. 11 No. 1 (2021)

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