Evaluating the Effectiveness of Traditional and Vitek Systems in the Diagnosis of Bacterial Urinary Tract Infections in Mosul

Authors

  • huda ali جامعة تكريت_ كلية العلوم قسم علوم الحياة
  • Waqas Saadi Mahmood Department of Biology, College of Science, University of Tikrit

DOI:

https://doi.org/10.32792/jeps.v15i4.742

Keywords:

UTIs, VITEK, conventional methods, antibiotic susceptibility

Abstract

   Urinary tract infections (UTIs) are among the most common bacterial infections worldwide, caused by a variety of pathogens, most notably Escherichia coli, Klebsiella spp., Proteus spp., Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus haemolyticus, and Staphylococcus hominis. To make sure that patients with UTIs getting the right antibiotics, point out the correct bacteria causing the infection and understanding their resistance is vital. While conventional procedures involve bacterial culturing and carrying out biochemical tests, automated systems like VITEK provide a faster, more technologically modern approach. This research compares the conventional and VITEK procedures in recognizing UTIs, looking at how speedily and precisely they work, in addition to their cost-effectiveness.

References

[ 1] Y. He, J. Zhao, L. Wang, C. Han, R. Yan, P. Zhu, et al., “Epidemiological trends and predictions of urinary tract infections in the global burden of disease study 2021,” Sci. Rep., vol. 15, no. 1, p. 4702, 2025, doi: 10.1038/s41598-025-51960-2.

[ 2] G. Mancuso, A. Midiri, E. Gerace, M. Marra, S. Zummo, and C. Biondo, “Urinary tract infections: The current scenario and future prospects,” Pathogens, vol. 12, no. 4, p. 623, 2023, doi: 10.3390/pathogens12040623.

[ 3] A. L. Flores-Mireles, J. N. Walker, M. Caparon, and S. J. Hultgren, “Urinary tract infections: Epidemiology, mechanisms of infection and treatment options,” Nat. Rev. Microbiol., vol. 22, no. 3, pp. 123–137, 2024, doi: 10.1038/s41579-024-00892-1.

[ 4] N. J. Zhu, M. Weldegiorgis, E. Carter, C. Brown, A. Holmes, and P. Aylin, “Economic burden of community-acquired antibiotic-resistant urinary tract infections: Systematic review and meta-analysis,” JMIR Public Health Surveill., vol. 10, p. e53828, 2024, doi: 10.2196/53828.

[ 5] R. Singh, M. Gupta, and A. Sharma, “Comparative evaluation of automated VITEK 2 and conventional methods in identification and antimicrobial susceptibility testing of uropathogens,” J. Clin. Diagn. Res., vol. 17, no. 4, pp. DC01–DC05, 2023, doi: 10.7860/JCDR/2023/59231.16743.

[ 6] Clinical and Laboratory Standards Institute, Performance standards for antimicrobial susceptibility testing, 33rd ed. Wayne, PA: CLSI, 2023.

[ 7] H. Al Lawati, B. M. Blair, and J. Larnard, “Urinary tract infections: Core curriculum 2024,” Am. J. Kidney Dis., vol. 83, no. 1, pp. 90–100, 2024, doi: 10.1053/j.ajkd.2023.08.009.

[ 8] A. Irvine, J. Watt, M. J. Kurth, J. V. Lamont, P. Fitzgerald, and M. W. Ruddock, “The importance of diagnostics in the treatment of urinary tract infections,” Res. Rep. Urol., vol. 16, pp. 1–9, 2024, doi: 10.2147/RRU.S483147.

[ 9] S. Kumar, R. Singh, and A. Sharma, “Antibiotic susceptibility testing: An essential tool for effective antimicrobial therapy,” J. Med. Microbiol., vol. 72, no. 4, p. 001695, 2023, doi: 10.1099/jmm.0.001695.

[ 10] M. H. Ali, S. M. Kareem, and R. H. Hassan, “Identification and antimicrobial susceptibility of uropathogens using VITEK 2 compact system in Wasit Governorate, Iraq,” Int. J. Med. Microbiol. Res., vol. 11, no. 2, pp. 45–52, 2023.

[ 11] L. Jones and R. Miller, “Use of nitrogen agar for short-term storage of microbial samples,” Int. J. Microbiol. Methods, vol. 58, no. 3, pp. 150–158, 2021.

[ 12] Clinical and Laboratory Standards Institute, Performance standards for antimicrobial susceptibility testing, 34th ed. Wayne, PA: CLSI, 2024.

[ 13] H. Dawoodi, “Estimation of some natural products of the plants Tanoum and Marrar and their effect in inhibiting the growth of bacteria causing urinary tract infections,” Ph.D. dissertation, Univ. Tikrit, Tikrit, Iraq, 2022.

[ 14] Z. M. Karim, “Isolation and identification of bacteria causing urinary tract infections with phenotypic and genotypic detection of some resistance mechanisms in Escherichia coli,” M.S. thesis, Univ. Tikrit, Tikrit, Iraq, 2022.

[ 15] T. M. Hooton and K. Gupta, “Urinary tract infections and asymptomatic bacteriuria in women,” Nat. Rev. Urol., vol. 19, no. 3, pp. 157–170, 2022, doi: 10.1038/s41585-021-00534-w.

[ 16] H. Rubi, G. Mudey, and R. Kunjalwar, “Catheter-associated urinary tract infection (CAUTI),” Cureus, vol. 14, no. 10, p. e30804, 2022, doi: 10.7759/cureus.30804.

[ 17] Centers for Disease Control and Prevention, “Catheter-associated urinary tract infections (CAUTI),” CDC, Atlanta, GA, 2024.

[ 18] H. Kaitan and M. T. Fleyh, “Prevalence of swarming genes in Escherichia coli isolates from urinary tract infections and catheter-associated urinary tract infections,” J. Coll. Med. – Univ. Baghdad, vol. 66, no. 4, pp. 123–130, 2024.

[ 19] C. Preda, D. Dragomir, A. Enache, A. Călin, and D. C. Badiu, “Predictive factors for urinary tract infections in patients with type 2 diabetes,” J. Clin. Med., vol. 13, no. 24, p. 7628, 2024, doi: 10.3390/jcm13247628.

[ 20] N. Gremke, K. Kostev, and M. Kalder, “Association between antihypertensive medication and the risk of urinary tract infection (UTI) of outpatients: A retrospective cohort study,” Infection, 2022, doi: 10.1007/s15010-022-01895-8.

[ 21] G. Wang, Y. Zhu, S. Feng, B. Wei, Y. Zhang, J. Wang, et al., “Extended-spectrum beta-lactamase-producing Enterobacteriaceae related urinary tract infection in adult cancer patients: A multicenter retrospective study, 2015–2019,” BMC Infect. Dis., vol. 23, no. 1, p. 129, 2023, doi: 10.1186/s12879-023-08008-3.

[ 22] S. Khemiri, S. Masmoudi, S. Mezghanni, W. B. Kridis, A. Hammami, and K. Afef, “Urinary tract infections in patients with solid tumors: Retrospective study,” J. Clin. Nephrol. Renal Care, vol. 8, no. 2, 2022, doi: 10.23937/2572-3286.1510075.

[ 23] M. D. Silverman and M. A. Turrentine, “Urinary tract infections in pregnant individuals,” in Textbook of Obstetrics and Gynecology. Amsterdam, The Netherlands: Elsevier, 2023, pp. 345–350.

[ 24] American College of Obstetricians and Gynecologists, “Urinary tract infections in pregnant individuals,” in Textbook of Obstetrics and Gynecology. Amsterdam, The Netherlands: Elsevier, 2023, pp. 345–350.

[ 25] S. N. Darwish, “Immunological changes of some indicators associated with bacterial species causing urinary tract infections in pregnant women,” M.S. thesis, Univ. Tikrit, Tikrit, Iraq, 2021.

[ 26] A. M. Darwesh, “Bacteriological study of urinary tract infections in elderly patients,” M.S. thesis, Univ. Mosul, Mosul, Iraq, 2021.

[ 27] Z. Hussein, “Bacteriological and molecular study of urinary tract infections among patients in Mosul,” M.S. thesis, Univ. Mosul, Mosul, Iraq, 2021.

[ 28] C. M. Kunin, “Urinary tract infections: Clinical features and microbiology,” in Goldman-Cecil Medicine, 26th ed., L. Goldman and A. I. Schafer, Eds. Amsterdam, The Netherlands: Elsevier, 2023.

[ 29] M. Jabar, “Identification of uropathogens and their antimicrobial resistance patterns,” M.S. thesis, Univ. Baghdad, Baghdad, Iraq, 2014.

[ 30] S. Kareem, “Urinary tract infections in children and their resistance patterns,” M.S. thesis, Univ. Kirkuk, Kirkuk, Iraq, 2021.

[ 31] M. Sohail, M. Khurshid, H. G. Saleem, H. Javed, and A. A. Khan, “Characteristics of biofilm producing Staphylococcus aureus from urinary tract infections and their antimicrobial resistance,” Pak. J. Med. Sci., vol. 31, no. 1, pp. 105–110, 2015.

[ 32] M. Yousefi, M. R. Pourmand, and F. Fallah, “Biofilm formation and virulence genes expression in Staphylococcus aureus isolated from medical devices,” Iran. J. Pathol., vol. 11, no. 4, pp. 313–320, 2016.

[ 33] L. Ibrahim, “Study of Gram-positive cocci isolated from urinary tract infections in children,” M.S. thesis, Univ. Tikrit, Tikrit, Iraq, 2020.

[ 34] Z. Najjar, “Bacterial causes of urinary tract infections in Tikrit City and their resistance profile,” Tikrit J. Pure Sci., vol. 25, no. 2, pp. 102–110, 2020.

[ 35] R. Ismael and A. Atiyea, “Incidence and antibiotic resistance pattern of uropathogenic bacteria in Mosul City,” J. Educ. Sci., vol. 30, no. 4, pp. 45–54, 2021, doi: 10.33899/edusj.2021.129614.1142.

[ 36] A. Musa, “Prevalence and antimicrobial resistance of Escherichia coli among UTI patients in Kirkuk,” M.S. thesis, Univ. Kirkuk, Kirkuk, Iraq, 2021.

[ 37] A. Hamza and S. Fazaa, “Prevalence and antimicrobial resistance pattern of uropathogens in Baghdad hospitals,” Iraqi Ministry Health Publ., 2023.

[ 38] M. Mohammed and H. Qaddoura, “Urinary tract infections: Causes, diagnosis, and treatment in clinical settings,” Baghdad Med. J., vol. 66, no. 1, pp. 22–30, 2024.

[ 39] J. Sarowska, B. Futoma-Koloch, and A. Jama-Kmiecik, “Virulence factors, prevalence and resistance patterns of uropathogenic Escherichia coli,” Adv. Clin. Exp. Med., vol. 28, no. 9, pp. 1287–1297, 2019, doi: 10.17219/acem/104529.

[ 40] S. Kareem, “Prevalence of multidrug-resistant bacteria in urinary tract infections among hospitalized patients in Kirkuk,” Kirkuk Univ. J. Sci. Stud., vol. 15, no. 1, pp. 85–93, 2020.

[ 41] B. Al-Tikrity, “Bacteriological study of urinary tract infection in Erbil City,” M.S. thesis, Univ. Salahaddin, Erbil, Iraq, 2016.

[ 42] R. Ismael, “Incidence and antibiotic resistance pattern of uropathogenic bacteria in Mosul City,” J. Educ. Sci., vol. 30, no. 4, pp. 45–54, 2021.

[ 43] S. Al-Aani, “The prevalence of Proteus species in UTI patients in Fallujah hospitals,” M.S. thesis, Univ. Anbar, Anbar, Iraq, 2018.

[ 44] Alsaffar and A. Jaralla, “Bacteriological and antibiotic susceptibility profile of urinary tract infections in Diyala province,” Iraqi J. Sci., vol. 60, no. 9, pp. 1921–1930, 2019, doi: 10.24996/ijs.2019.60.9.10.

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Published

2025-12-02